Recombinant Bovine Fibroblast growth factor 2 (FGF2) (Active)
Product Specifications
CAS Number
9000-83-3
Gene Name
bFGF/FGF-2
UniProt
P03969
Expression Region
10-155aa
Organism
Bos taurus
Target Sequence
PALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYSSWYVALKRTGQYKLGPKTGPGQKAILFLPMSAKS
Tag
C-terminal 6xHis-tagged
Source
E coli
Field of Research
Others
Assay Type
Active Protein & In Stock Protein
Endotoxin
≤100 EU/mg by the LAL method
Purity
Greater than 95% as determined by SDS-PAGE.
Activity
Yes
Bioactivity
Measured in a cell proliferation assay using NIH3T3 mouse embryonic fibroblast cells. The ED50 for this effect is ≤2.0 ng/mL.
Length
Full Length of Mature Protein
Form
Lyophilized powder
Buffer
Lyophilized from a 0.2 μm filtered solution containing 10mM Tris, 200mMNacl, 5% mannitol, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Molecular Weight
17.4 kDa
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Available Sizes
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