Recombinant Bovine Fibroblast growth factor 2 (FGF2) (Active)

Product Specifications

CAS Number

9000-83-3

Gene Name

bFGF/FGF-2

UniProt

P03969

Expression Region

10-155aa

Organism

Bos taurus

Target Sequence

PALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYSSWYVALKRTGQYKLGPKTGPGQKAILFLPMSAKS

Tag

C-terminal 6xHis-tagged

Source

E coli

Field of Research

Others

Assay Type

Active Protein & In Stock Protein

Endotoxin

≤100 EU/mg by the LAL method

Purity

Greater than 95% as determined by SDS-PAGE.

Activity

Yes

Bioactivity

Measured in a cell proliferation assay using NIH3T3 mouse embryonic fibroblast cells. The ED50 for this effect is ≤2.0 ng/mL.

Length

Full Length of Mature Protein

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 μm filtered solution containing 10mM Tris, 200mMNacl, 5% mannitol, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

17.4 kDa

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.