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MUC1 Antibody

In Western blotting, it recognizes proteins in MW range of 265-400kDa, identified as different glycoforms of EMA. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinomas. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL

UniProt

P15941

Host

Mouse

Reactivity

Human

Immunogen

Delipidated extract of human milk fat globule membranes was used as the immunogen for this MUC1 antibody.

Clonality

Monoclonal

Isotype

IgG2a λ

Clone

E29

Applications

IHC-P, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the MUC1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This MUC1 antibody is available for research use only.

Storage Conditions

Store the MUC1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Location

Cytoplasmic & cell surface

Image Legend

Western blot testing of human T-47D cell lysate with MUC1 antibody (clone E29) . This glycoprotein is commonly visualized between 120~500 kDa.

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