MUC1 Antibody
In Western blotting, it recognizes proteins in MW range of 265-400kDa, identified as different glycoforms of EMA. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinomas. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL
UniProt
P15941
Host
Mouse
Reactivity
Human
Immunogen
Delipidated extract of human milk fat globule membranes was used as the immunogen for this MUC1 antibody.
Clonality
Monoclonal
Isotype
IgG2a λ
Clone
E29
Applications
IHC-P, WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the MUC1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This MUC1 antibody is available for research use only.
Storage Conditions
Store the MUC1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cytoplasmic & cell surface
Image Legend
Western blot testing of human T-47D cell lysate with MUC1 antibody (clone E29) . This glycoprotein is commonly visualized between 120~500 kDa.
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