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BrdU Antibody

It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

UniProt

Not Applicable

Host

Mouse

Reactivity

All Species Expected

Immunogen

Bromodeoxyuridine was used as the immunogen for this BrdU antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

BU20a

Applications

FACS, IF, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the BrdU antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This BrdU antibody is available for research use only.

Storage Conditions

Store the BrdU antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Nuclear

Image Legend

IHC: Formalin-fixed, paraffin-embedded mouse small intestine stained with BrdU antibody (BU20a) .

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