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Bcl-X Antibody Cocktail

Recognizes a protein of 27kDa, identified as the Bcl-X protein. This mAb cocktail shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This mAb reacts with both Bcl-XS and Bcl-XL proteins.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

UniProt

Q07817

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 3-14 (QSNRELVVDFLS) from the human protein were used as the immunogen for this Bcl-X antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

BX006 + 2H12

Applications

WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Bcl-X antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Bcl-X antibody cocktail is available for research use only.

Storage Conditions

Store the Bcl-X antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic and cell/nuclear membrane

Image Legend

IHC: Formalin-paraffin Hodgkin's lymphoma stained with Bcl-x antibody (BX006 + 2H12) .

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