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Acidic Cytokeratin Antibody

This mAb recognizes the 56.5kDa (CK10) ; 50kDa (CK14) ; 50kDa (CK15) ; 48kDa (CK16) ; 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Product Specifications

CAS Number

9007-83-4

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

UniProt

Q7Z794

Host

Mouse

Reactivity

Human, Rat

Immunogen

Recombinant human KRT77 protein was used as the immunogen for the Acidic Cytokeratin antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

KRTL/1077

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Reconstitution

Store the Acidic Cytokeratin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Acidic Cytokeratin antibody is available for research use only.

Storage Conditions

Store the Acidic Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Location

Cytoplasmic

Image Legend

IHC: Formalin paraffin human skin stained with Acidic Cytokeratin antibody (KRTL/1077) .

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