BrdU Antibody
It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Product Specifications
CAS Number
9007-83-4
Specifications
UniProt
Not Applicable
Host
Mouse
Reactivity
All Species Expected
Immunogen
Bromodeoxyuridine conjugated to hemocyanine was used as the immunogen for the BrdU antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
85-2C8
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Reconstitution
Store the BrdU antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This BrdU antibody is available for research use only.
Storage Conditions
Store the BrdU antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*
Location
Nuclear
Image Legend
IHC: Formalin-fixed, paraffin-embedded mouse small intestine stained with BrdU antibody (85-2C8) .
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