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BrdU Antibody

It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Product Specifications

CAS Number

9007-83-4

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

UniProt

Not Applicable

Host

Mouse

Reactivity

All Species Expected

Immunogen

Bromodeoxyuridine conjugated to hemocyanine was used as the immunogen for the BrdU antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

85-2C8

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Reconstitution

Store the BrdU antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This BrdU antibody is available for research use only.

Storage Conditions

Store the BrdU antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Product Datasheet

https://www.nsjbio.com/tds-pdf/brdu-antibody-85-2c8-v3112

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Location

Nuclear

Image Legend

IHC: Formalin-fixed, paraffin-embedded mouse small intestine stained with BrdU antibody (85-2C8) .

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