BrdU Antibody
It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT
UniProt
Not Applicable
Host
Mouse
Reactivity
All Species Expected
Immunogen
Bromodeoxyuridine conjugated to hemocyanine was used as the immunogen for the BrdU antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
85-2C8
Applications
FACS, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the BrdU antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This BrdU antibody is available for research use only.
Storage Conditions
Store the BrdU antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Nuclear
Image Legend
IHC: Formalin-fixed, paraffin-embedded mouse small intestine stained with BrdU antibody (85-2C8) .
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