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Nuclear Marker Antibody

This mAb is an excellent marker for all nuclei in cells in tissues. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This mAb recognizes an antigen associated with the nuclei in all cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed tissue sections.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Not Known

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Nuclei of HL60 cells were used as the immunogen for the Nuclear Marker antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

NM106

Applications

IF, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Nuclear Marker antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Nuclear Marker antibody is available for research use only.

Storage Conditions

Store the Nuclear Marker antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Nuclei

Image Legend

IHC: Formalin-fixed, paraffin-embedded human tonsil stained with Nuclear Marker antibody (clone NM106) .

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