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Helicobacter pylori Antibody

The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. H. pylori is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA: 1-5 µg/mL for coating (order BSA/sodium azide-free format), Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2 µg/mL

UniProt

Not Known

Host

Mouse

Reactivity

Helicobacter pylori

Immunogen

Total sonicate of Helicobacter pylori was used as the immunogen for the Helicobacter pylori antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

B-53.4

Applications

ELISA, FACS, IF

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the Helicobacter pylori antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Helicobacter pylori antibody is available for research use only.

Storage Conditions

Store the Helicobacter pylori antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the Helicobacter pylori antibody should be determined by the researcher.

Location

Cell surface and cytoplasm

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