Neurofilament Antibody
This mAb reacts with a 200kDa and 68kDa protein, identified as heavy and light sub-units of neurofilaments (NF-H & NF-L) . Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H) . Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and cell carcinomas of the lung also express neurofilament.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 0.25-0.5 µg/mL for 30 min at RT
UniProt
P12036
Host
Mouse
Reactivity
Human
Immunogen
Semi-purified human neurofilaments from spinal cord were used as the immunogen for the Neurofilament antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
SPM145
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Neurofilament antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Neurofilament antibody is available for research use only.
Storage Conditions
Store the Neurofilament antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cytoplasmic, membranous
Image Legend
IHC: Formalin-fixed, paraffin-embedded human cerebellum stained with Neurofilament antibody (SPM145) .
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