Pan Muscle Actin Antibody
This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas) .
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P62736, P68032, P68133
Host
Mouse
Reactivity
Human, Rat
Immunogen
An amino acid sequence common among human skeletal, cardiac and smooth muscle actins was used as the immunogen for the pan Muscle Actin antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
MSA/953
Applications
WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the pan Muscle Actin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This pan Muscle Actin antibody is available for research use only.
Storage Conditions
Store the pan Muscle Actin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cytoplasmic
Image Legend
IHC: Formalin-fixed, paraffin-embedded human rhabdomyosarcoma stained with pan Muscle Actin antibody (clone MSA/953) .
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