DsDNA Antibody

This mAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. This mAb produces a homogeneous staining pattern in the nucleus of normal and malignant cells. Double Stranded deoxyribonucleic acid (ds DNA) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3',5'-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds between complementary base pairs.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow Cytometry: 1-2ug/million cells in 0.1ml, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT (1)

UniProt

Not Known

Host

Mouse

Reactivity

Human

Immunogen

Nuclei of Burkitt's cells were used as the immunogen for the dsDNA antibody.

Clonality

Monoclonal

Isotype

IgG3 κ

Clone

AE-2

Applications

FACS, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the dsDNA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This dsDNA antibody is available for research use only.

Storage Conditions

Store the dsDNA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the dsDNA antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Location

Nuclear

Image Legend

IHC staining of FFPE human colon carcinoma with dsDNA antibody (clone AE-2) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.