Phosphotyrosine Antibody (p-Tyr)

Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This mAb shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Not Applicable

Host

Mouse

Immunogen

P-Tyr conjugated to BSA was used as the immunogen for the Phosphotyrosine antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

PY793

Applications

FACS, IF, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Phosphotyrosine antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Phosphotyrosine antibody is available for research use only.

Storage Conditions

Store the Phosphotyrosine antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Phosphotyrosine antibody should be determined by the researcher.1. No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues