H pylori Antibody
The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. H. pylori is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.
Product Specifications
CAS Number
9007-83-4
Specifications
ELISA: 1-5 µg/mL for coating (order BSA/sodium azide-free format), Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2 µg/mL
UniProt
Not Known
Host
Mouse
Reactivity
Helicobacter pylori
Immunogen
Total sonicate of H pylori was used as the immunogen for the H pylori antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
HP/212
Applications
ELISA, FACS, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the H pylori antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This H pylori antibody is available for research use only.
Storage Conditions
Store the H pylori antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the H pylori antibody should be determined by the researcher.
Location
Cell surface and cytoplasm
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