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Multi Cytokeratin Antibody

Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This mAb recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59kDa (CK4) ; 58kDa (CK5) ; 56kDa (CK6) ; 52kDa (CK8) ; 56.5kDa (CK10) ; 53kDa (CK13) and 45kDa (CK18) . This is a broad-spectrum mAb which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Product Specifications

CAS Number

9007-83-4

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

UniProt

Multi

Host

Mouse

Reactivity

Human, Rat

Immunogen

A Keratin-enriched preparation from cultured human epithelial cells was used as the immunogen for the Multi Cytokeratin antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

KRT/457

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Reconstitution

Store the Multi Cytokeratin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Multi Cytokeratin antibody is available for research use only.

Storage Conditions

Store the Multi Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Location

Cytoplasmic

Image Legend

IHC: Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Multi Cytokeratin antibody (KRT/457) .

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