HCG Holo Antibody
This mAb reacts only with the intact-HCG and not with either free alpha- or free beta-chain of HCG. </strong>HCG is a glycoprotein and is composed of two non-identical, non-covalently linked polypeptide chains designated as the alpha and beta subunits. The alpha subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH) . HCG is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG mAb detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate anti-hCG positivity in 90% and 60% of cases respectively. 20% of lung squamous cell carcinomas are positive. HCG expression by non-trophoblastic tumors may indicate aggressive behavior.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT
UniProt
P01215, P0DN86
Host
Mouse
Reactivity
Human
Immunogen
Purified hCG protein was used as the immunogen for the hCG Holo antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
HCGab/52
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the hCG Holo antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This hCG Holo antibody is available for research use only.
Storage Conditions
Store the hCG Holo antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cytoplasmic, secreted
Image Legend
IHC: Formalin-fixed, paraffin-embedded human placenta stained with hCG Holo antibody (clone HCGab/52) .
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