HPV-16 Antibody

Reacts with a protein of 57kDa, identified as the L1 protein of human papilloma virus type 16 (HPV-16) . It is the major capsid protein of HPV-16. Infection with specific types of HPV has been associated with an increased risk of developing cervical neoplasia. HPV types 6 and 11 have been associated with relatively benign diseases such as genital warts but types 16 and 18 are strongly associated with cervical, vaginal, and vulvar malignancies. The antibody reacts very strongly with formalin-fixed, paraffin-embedded tissues containing HPV-16 or -33; very weak reactions were occasionally observed with biopsy specimens or smears containing HPV-6 or HPV-11. It cross-reacts with HPV37.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P03101

Host

Mouse

Reactivity

HPV-16

Immunogen

Recombinant major capsid protein L1 from human papilloma virus type 16 was used as the immunogen for the HPV-16 antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

SPM405

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the HPV-16 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This HPV-16 antibody is available for research use only.

Storage Conditions

Store the HPV-16 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the HPV-16 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.