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L1 Antibody

The antibody reacts very strongly with formalin-fixed, paraffin-embedded tissues containing HPV-16 or -33; very weak reactions were occasionally observed with biopsy specimens or smears containing HPV-6 or HPV-11. It cross-reacts with HPV37.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

UniProt

P03101

Host

Mouse

Reactivity

HPV-16

Immunogen

Human papilloma virus type 16, major capsid protein L1, was used as the immunogen for the L1 antibody. The epitope has been localized to AA 204-210. (Ref 1)

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

CamVir-1

Applications

IF, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the L1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This L1 antibody is available for research use only.

Storage Conditions

Store the L1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the L1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

Location

Nuclear

Image Legend

IHC testing of formalin-fixed, paraffin-embedded human cervix with L1 antibody (clone CamVir-1)

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