Cytokeratin 5/8 Antibody
It reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa) . Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P13647, P05787
Host
Mouse
Reactivity
Human
Immunogen
A cytoskeletal preparation from HeLa cells was used as the immunogen for the Cytokeratin 5/8 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
SPM268
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Cytokeratin 5/8 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Cytokeratin 5/8 antibody is available for research use only.
Storage Conditions
Store the Cytokeratin 5/8 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cytoplasmic
Image Legend
IHC: Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cytokeratin 5/8 antibody (SPM268) .
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