Cytokeratin 5/8 Antibody

It reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa) . Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL, Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

UniProt

P13647, P05787

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

A cytoskeletal preparation from HeLa cells was used as the immunogen for the Cytokeratin 5/8 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

C-50

Applications

FACS, IF, WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Cytokeratin 5/8 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Cytokeratin 5/8 antibody is available for research use only.

Storage Conditions

Store the Cytokeratin 5/8 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Cytokeratin 5/8 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Location

Cytoplasmic

Image Legend

IHC analysis of formalin-fixed, paraffin-embedded human colon carcinoma stained with Cytokeratin 5/8 antibody (clone C-50) . Required HIER: digest sections with Trypsin at 1 mg/mL, 15 min, at RT.