Blood Group Lewis a Antibody
Recognizes a carbohydrate determinant of Gal 1-3 (Fuc 1-4) GlcNAc which is blood group antigen Lewis A. It is present primarily on epithelial cells such as colon and kidneys. In the tumors and dedifferentiated tissues, decrease of Lewis A antigen was observed. Lewis A (type 1 chain) is expressed in colonic epithelial cells and may be useful for detection of gastrointestinal tumors, pancreatic cancer, and colorectal tumors. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT
UniProt
Not Applicable
Host
Mouse
Reactivity
Human, Mouse
Immunogen
Mucins isolated from ovarian cyst fluid were used as the immunogen for the Blood Group Lewis a antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
7LE
Applications
FACS, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Blood Group Lewis a antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Blood Group Lewis a antibody is available for research use only.
Storage Conditions
Store the Blood Group Lewis a antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cell membrane, cytoplasmic and extracellular
Image Legend
IHC: Formalin-fixed, paraffin-embedded human colon carcinoma stained with Blood Group Lewis a antibody (clone 7LE) .
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