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Cytokeratin 10/13 Antibody

This antibody recognizes cytokeratin 10 (56.5kDa) and cytokeratin 13 (53kDa) in western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this mAb serves as differentiation-related marker of all stratified epithelia; it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P13645, P13646

Host

Mouse

Reactivity

Human

Immunogen

A cytoskeletal preparation extracted from human ectocervical epithelium was used as the immunogen for the Cytokeratin 10/13 antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

SPM262

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Cytokeratin 10/13 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Cytokeratin 10/13 antibody is available for research use only.

Storage Conditions

Store the Cytokeratin 10/13 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic

Image Legend

IHC analysis of formalin-fixed, paraffin-embedded human tonsil stained with Cytokeratin 10/13 antibody (clone SPM262) .

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