GITR Antibody (TNFRSF18)
GITR (glucocorticoid-induced TNFR-related gene) is a member of the TNF-receptor superfamily, also known as TNFRSF18. It is expressed at low levels on resting T lymphocytes and at high levels on CD25+ CD4+ Tregs. The expression of GITR on T cells can be upregulated upon activation. Interaction of GITR with its ligand (GITRL) has been demonstrated to augment T cell activation, proliferation, cytokine production as well as MAPKs and NF-kB activation, and abrogate the inhibitory function of CD25+ CD4+ Tregs. In vivo activation of GITR causes development of autoimmune diseases and restores the suppressed immune response.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL
UniProt
O35714 (Mouse)
Host
Rat
Reactivity
Mouse
Immunogen
Mouse CD25+ CD4+ T cells were used as the immunogen for the GITR antibody.
Clonality
Monoclonal
Isotype
IgG2b λ
Clone
DTA-1
Applications
FACS, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the GITR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This GITR antibody is available for research use only.
Storage Conditions
Store the GITR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the GITR antibody should be determined by the researcher.
Location
Secreted, cell surface
Image Legend
SDS-PAGE Analysis of Purified, BSA-Free GITR Antibody (clone DTA-1) . Confirmation of Integrity and Purity of the Antibody.
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