MVP Antibody
Recognizes a protein of 104kDa-110kDa, characterized as major vault protein (MVP) . Vaults are large ribonucleoprotein particles (RNPs) present in all eukaryotic cells. They have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for >70% of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
Q14764
Host
Mouse
Reactivity
Human
Immunogen
Proteins precipitated from human breast cancer MCF-7 cells were used as the immunogen for the MVP antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
SPM280
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the MVP antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This MVP antibody is available for research use only.
Storage Conditions
Store the MVP antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Location
Cytoplasmic, nuclear
Image Legend
IHC: Formalin-fixed, paraffin-embedded human testicular carcinoma stained with MVP antibody (clone SPM280) .
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