Major Vault Protein Antibody
Recognizes a protein of 104kDa-110kDa, characterized as major vault protein (MVP) . Vaults are large ribonucleoprotein particles (RNPs) present in all eukaryotic cells. They have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for >70% of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunofluorescence: 1-2 µg/mL
UniProt
Q14764
Host
Mouse
Reactivity
Human
Immunogen
Proteins precipitated from human breast cancer MCF-7 cells were used as the immunogen for the Major Vault Protein antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
1014
Applications
WB, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Major Vault Protein antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Major Vault Protein antibody is available for research use only.
Storage Conditions
Store the Major Vault Protein antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the Major Vault Protein antibody should be determined by the researcher.
Location
Cytoplasmic, nuclear
Image Legend
Immunofluorescent staining of human A549 cells with Major Vault Protein antibody (green, clone 1014) and Reddot nuclear stain (red) .
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