CDC2 Antibody / CDK1

Recognizes a 34kDa protein, which is identified as cyclin dependent kinase 1 (cdk1) or p34cdc2 protein kinase. Cdc2 plays a crucial role during cell division and is most active during mitosis. It is predominantly localized in the nucleus. It is a serine/threonine kinase, which is activated by cyclin, presumably by de-phosphorylation of tyrosine residues. Activated Cdc2 performs specific functions during mitosis, including nuclear envelope breakdown and chromosome condensation.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 2-4 µg/mL for 30 min at RT, Western blot: 1-2 µg/mL

UniProt

P06493

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human protein was used as the immunogen for the CDC2 antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

CDK1/873

Applications

IHC-P, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the CDC2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CDC2 antibody is available for research use only.

Storage Conditions

Store the CDC2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the CDC2 antibody should be determined by the researcher.

Location

Nuclear and cytoplasmic

Image Legend

IHC testing of formalin-fixed, paraffin-embedded human tonsil with CDC2 antibody (CDK1/873) . Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.