CD59 Antibody
Reacts with human CD59, a 20kDa glycosyl phosphatidyl-inositol (GPI) -anchored cell surface protein. CD59 regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. CD59 is widely distributed on cells in all tissues. It inhibits formation of MAC, thus protecting cells from complement-mediated lysis. The expression of CD59 on erythrocytes is important for their survival. Genetic defects in GPI-anchor attachment, that cause a reduction or loss of CD59 and CD55 on erythrocytes produce the symptoms of the disease paroxysmal hemoglobinuria (PNH) . This mAb is useful for study on GPI-anchored proteins, PNH and CD59 functions.
Product Specifications
CAS Number
9007-83-4
Specifications
Functional studies (order BSA/sodium azide-free format), Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL
UniProt
P13987
Host
Mouse
Reactivity
Human
Immunogen
Human K562 tumor cells were used as the immunogen for the CD59 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
BRA-10G
Applications
Functional studies, FACS, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the CD59 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD59 antibody is available for research use only.
Storage Conditions
Store the CD59 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the CD59 antibody should be determined by the researcher.
Location
Plasma membrane, cytoplasm
Image Legend
SDS-PAGE Analysis of Purified, BSA-Free CD59 Antibody (clone BRA-10G) . Confirmation of Integrity and Purity of the Antibody.
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