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CD48 Antibody

Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI) -anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of the markers for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL

UniProt

P09326

Host

Mouse

Reactivity

Human

Immunogen

Human peripheral blood lymphocytes were used as the immunogen for the CD48 antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

5-4.8

Applications

FACS, IF

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the CD48 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CD48 antibody is available for research use only.

Storage Conditions

Store the CD48 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the CD48 antibody should be determined by the researcher.

Location

Cell surface

Image Legend

SDS-PAGE Analysis of Purified, BSA-Free CD48 Antibody (clone 5-4.8) . Confirmation of Integrity and Purity of the Antibody.

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