CD48 Antibody
Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI) -anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of the markers for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL
UniProt
P09326
Host
Mouse
Reactivity
Human
Immunogen
Human peripheral blood lymphocytes were used as the immunogen for the CD48 antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
5-4.8
Applications
FACS, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the CD48 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD48 antibody is available for research use only.
Storage Conditions
Store the CD48 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the CD48 antibody should be determined by the researcher.
Location
Cell surface
Image Legend
SDS-PAGE Analysis of Purified, BSA-Free CD48 Antibody (clone 5-4.8) . Confirmation of Integrity and Purity of the Antibody.
Frequently Asked Questions
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