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CD47 Antibody Cocktail

This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL

UniProt

Q08722

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human protein (IAP/964) and intact CD47 purified from placenta (B6H12.2) were used as the immunogen for the CD47 antibody cocktail.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

IAP/964 + B6H12.2

Applications

FACS, IF

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the CD47 antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CD47 antibody cocktail is available for research use only.

Storage Conditions

Store the CD47 antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the CD47 antibody cocktail should be determined by the researcher.

Location

Cell surface, cytoplasmic

Image Legend

Immunofluorescent staining of PFA-fixed human MCF-7 cells with CD47 antibody cocktail (green, clones B6H12.2 + IAP/964) and Reddot nuclear stain (red) .

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