CD47 Antibody / IAP
This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunofluorescence: 1-2 µg/mL, Flow cytometry: 1-2ug/million cells
UniProt
Q08722
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human protein was used as the immunogen for the CD47 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
IAP/964
Applications
IF, FACS
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the CD47 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD47 antibody is available for research use only.
Storage Conditions
Store the CD47 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the CD47 antibody should be determined by the researcher.
Location
Cell surface, cytoplasmic
Image Legend
Immunofluorescent staining of live human Jurkat cells with CD47 antibody (green, clone IAP/964) and Reddot nuclear stain (red) .
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