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Napsin A Antibody Cocktail

Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B. There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL for 60 min at RT, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

O96009

Host

Mouse

Reactivity

Human

Immunogen

Amino acids 189-299 from the human protein were used as the immunogen for the mAbs in the Napsin A antibody cocktail.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

NAPSA/1238 + NAPSA/1239

Applications

FACS, IF, WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Napsin A antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Napsin A antibody cocktail is available for research use only.

Storage Conditions

Store the Napsin A antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic

Image Legend

IHC: Formalin-fixed, paraffin-embedded human lung adenocarcinoma stained with Napsin-A antibody (NAPSA/1238 + NAPSA/1239) .

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