CD30 Antibody

Recognizes a single chain glycoprotein of 105/120kDa, identified as CD30/Ki-1. Its epitope is located between aa112-412. CD30 is synthesized as a 90kDa precursor, which is processed in the Golgi complex into a membrane-bound phosphorylated mature 105/120kDa glycoprotein. In Hodgkin s disease, CD30/Ki-1 antigen is expressed by mononuclear-Hodgkin and multinucleated Reed-Sternberg cells. It is also expressed by the tumor cells of a majority of anaplastic large cell lymphomas as well as by a varying proportion of activated T and B cells. This mAb distinguishes large cell lymphomas derived from activated lymphoid cells from histiocytic malignancies and lymphomas derived from resting and precursor lymphoid cells or from anaplastic carcinomas. About one third of the Ki-1 positive lymphomas lack the leukocyte common antigen (CD45) .

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1:50-1:100 for 30 min at RT (1)

UniProt

P28908

Host

Mouse

Reactivity

Human

Immunogen

A Co cell line established from a patient with Hodgkin s disease of T-cell lineage was used as the immunogen for the CD30 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

Ber-H2

Applications

IHC-P

Purity

Unpurified high titer supernatant

Format

Culture supernatant

Buffer

Bioreactor concentrate with 0.05% sodium azide

Reconstitution

Store the CD30 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CD30 antibody is available for research use only.

Storage Conditions

Store the CD30 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Bioreactor concentrate with 0.05% sodium azide

Applications Notes

Optimal dilution of the CD30 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.