CD28 Antibody
Recognizes a glycoprotein of 44-88kDa, which is identified as CD28. It is the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL Functional studies (order BSA/sodium azide-free format)
UniProt
P10747
Host
Mouse
Reactivity
Human, Mouse
Immunogen
Stimulated human leukocytes were used as the immunogen for the CD28 antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
204.12
Applications
FACS, IF Functional studies
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the CD28 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD28 antibody is available for research use only.
Storage Conditions
Store the CD28 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the CD28 antibody should be determined by the researcher.
Location
Cell surface
Image Legend
Immunofluorescent staining of human Jurkat cells with CD28 antibody (green, clone 204.12) and Reddot nuclear stain (red) .
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