CD6 Antibody
CD6 is a type I transmembrane glycoprotein that contains a 24-amino acid signal sequence, three extracellular scavenger receptor cysteine-rich (SRCR) domains, a membrane-spanning domain and a 44-amino acid cytoplasmic domain. The CD6 glycoprotein is tyrosine phosphorylated during TCR-mediated T cell activation. CD6 shows significant homology to CD5. CD6 is present on mature thymocytes, peripheral T cells and a subset of B cells. Antibodies to CD6 are used to deplete T cells from bone marrow transplants to prevent graft versus host disease.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/million cells, Immunofluorescence: 0.5-1 µg/mL
UniProt
P30203
Host
Mouse
Reactivity
Human, Mouse
Immunogen
A CD8+ cytotoxic T-cell clone was used as the immunogen for the CD6 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
SPV-L14
Applications
FACS, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the CD6 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD6 antibody is available for research use only.
Storage Conditions
Store the CD6 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the CD6 antibody should be determined by the researcher.
Location
Cell surface and cytoplasmic
Image Legend
Immunofluorescent staining of human MOLT-4 cells with CD6 antibody (green, clone SPV-L14) and Reddot nuclear stain (red) .
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