CD6 Antibody
CD6 is a type I transmembrane glycoprotein that contains a 24-amino acid signal sequence, three extracellular scavenger receptor cysteine-rich (SRCR) domains, a membrane-spanning domain and a 44-amino acid cytoplasmic domain. The CD6 glycoprotein is tyrosine phosphorylated during TCR-mediated T cell activation. CD6 shows significant homology to CD5. CD6 is present on mature thymocytes, peripheral T cells and a subset of B cells. Antibodies to CD6 are used to deplete T cells from bone marrow transplants to prevent graft versus host disease.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/million cells, Immunofluorescence: 0.5-1 µg/mL
UniProt
P30203
Host
Mouse
Reactivity
Human, Mouse
Immunogen
A CD8+ cytotoxic T-cell clone was used as the immunogen for the CD6 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
SPV-L14
Applications
FACS, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the CD6 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD6 antibody is available for research use only.
Storage Conditions
Store the CD6 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the CD6 antibody should be determined by the researcher.
Location
Cell surface and cytoplasmic
Image Legend
Immunofluorescent staining of human MOLT-4 cells with CD6 antibody (green, clone SPV-L14) and Reddot nuclear stain (red) .
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