CCNB1 Antibody / Cyclin B1
It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Immunofluorescence: 1-2 µg/mL
UniProt
P14635
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human full-length protein was used as the immunogen for the CCNB1 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
SPM619
Applications
IHC-P, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the CCNB1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CCNB1 antibody is available for research use only.
Storage Conditions
Store the CCNB1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cytoplasmic & nuclear
Image Legend
Immunofluorescent staining of PFA-fixed human HeLa cells with CCNB1 antibody (clone SPM619) .
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