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CAD Antibody Cocktail / CALD1 / Caldesmon (HMW)

Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.

Product Specifications

CAS Number

9007-83-4

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

UniProt

Q05682

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human protein (CALD1/820) and crude human uterus extract (h-CALD) were used as the immunogen for the CAD antibody cocktail.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

CALD1/820 + h-CALD

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Reconstitution

Store the CAD antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CAD antibody cocktail is available for research use only.

Storage Conditions

Store the CAD antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Location

Cytoplasmic

Image Legend

IHC: Formalin-fixed, paraffin-embedded human uterus stained with CAD antibody (CALD1/820 + h-CALD) .

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