CAD Antibody / CALD1 / Caldesmon (HMW)
Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This mAb recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 0.25-0.5 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL
UniProt
Q05682
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full-length human protein was used as the immunogen for the CAD antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
CALD1/820
Applications
IHC-P, WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the CAD antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CAD antibody is available for research use only.
Storage Conditions
Store the CAD antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cytoplasmic
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