ZAP70 Antibody Cocktail
ZAP70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene. ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P43403
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full-length human protein was used as the immunogen for the ZAP70 antibody cocktail.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
ZAP70/528 + 2F3.2
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the ZAP70 antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This ZAP70 antibody cocktail is available for research use only.
Storage Conditions
Store the ZAP70 antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cytoplasmic
Image Legend
IHC: Formalin-fixed, paraffin-embedded human tonsil stained with ZAP70 antibody (clones ZAP70/528 + 2F3.2) .
Documents
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
TCL1 (T-Cell Marker) (TCL1/2078), CF594 conjugate, 0.1mg/mL
Von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) (VWF/1859R), Biotin conjugate, 0.1mg/mL
p63 (TP63) (NM_001114979) Human Over-expression Lysate
