ZAP-70 Antibody

9007-83-4

Western blot: 1-2 µg/mL, Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

P43403

Mouse

Human

Recombinant human protein (including amino acids 1-254 and the SH2 domains) was used as the immunogen for the ZAP-70 antibody.

Monoclonal

IgG2a κ

2F3.2

FACS, IF, WB, IHC-P

Protein G affinity chromatography

Purified

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Store the ZAP-70 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

This ZAP-70 antibody is available for research use only.

Store the ZAP-70 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Cytoplasmic

Immunofluorescent staining of PFA-fixed human Jurkat cells with ZAP-70 antibody (green, clone 2F3.2) and Reddot nuclear stain (red) .