VEGF Antibody / VEGFA
This mAb recognizes proteins of 19-22kDa (reducing) and 38kDa-44kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF) . It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet derived growth factor (PDGF) . There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 4-8 µg/mL for 30 min at RT
UniProt
P15692
Host
Mouse
Reactivity
Human
Immunogen
Human recombinant protein was used as the immunogen for the VEGF antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
SPM225
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the VEGF antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This VEGF antibody is available for research use only.
Storage Conditions
Store the VEGF antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Location
Cytoplasmic, cell surface and extracellular (secreted)
Image Legend
IHC: Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with VEGF antibody (clone SPM225) .
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