VCAM1 Antibody
Recognizes a protein of 110kDa, identified as CD106 (also known as vascular cell adhesion molecule-1 (VCAM-1), L1CAM and INCAM-100) . It is a member of the Ig superfamily of adhesion molecules and is expressed at high levels on cytokine stimulated vascular endothelial cells, and at minimal levels on un-stimulated endothelial cells. It is also present on follicular and inter-follicular dendritic cells of lymph nodes, myoblasts, and some macrophages. L1CAM serves as a ligand for leukocyte integrin (VLA-4 or CD49d/CD29) and mediates cell adhesion of leukocytes to activated endothelium. It plays a role in various immunological and inflammatory responses. This mAb inhibits the binding of leukocytes to L1CAM on stimulated endothelial cells.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2 µg/mL
UniProt
P19320
Host
Mouse
Reactivity
Human
Immunogen
Activated human umbilical vein endothelial cells (HUVEC) were used as the immunogen for the VCAM1 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
B-K9
Applications
FACS, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the VCAM1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This VCAM1 antibody is available for research use only.
Storage Conditions
Store the VCAM1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the VCAM1 antibody should be determined by the researcher.
Location
Cytoplasmic, membrane
Image Legend
SDS-PAGE Analysis of Purified, BSA-Free VCAM1 Antibody (clone B-K9) . Confirmation of Integrity and Purity of the Antibody.
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