GRP94 Antibody / HSP90B1

Recognizes a protein of 94kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96) . Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90) . This mAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grps are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-3 µg/mL, Western blot: 2-4 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P14625

Host

Rat

Reactivity

Human, Mouse, Rat

Immunogen

Purified glucose regulated protein 94 from chicken oviducts was used as the immunogen for the GRP94 antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

9G10.F8.2

Applications

FACS, IF, WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the GRP94 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This GRP94 antibody is available for research use only.

Storage Conditions

Store the GRP94 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic and nuclear

Image Legend

IHC analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue stained with GRP94 antibody (clone 9G10.F8.2) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.