TLR4 Antibody (Toll Like Receptor 4)

This mAb reacts with human Toll-like receptor 2 (TLR4) . It is a member of the Toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Functional studies (order BSA/sodium azide-free format)

UniProt

O00206

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human protein was used as the immunogen for the TLR4 antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

TLR4/230

Applications

Functional studies

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the TLR4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This TLR4 antibody is available for research use only.

Storage Conditions

Store the TLR4 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the TLR4 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.