TTF-1 Antibody / NKX2.1
Recognizes a protein of 40kDa, identified as Thyroid transcription factor-1, also called NKX2.1. TTF-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. Anti-TTF-1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates. Loss of TTF-1 expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. TTF-1 reactivity is also seen in thyroid malignancies.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT
UniProt
P43699
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant protein was used as the immunogen for the TTF-1 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
NX2.1/690
Applications
FACS, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the TTF-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This TTF-1 antibody is available for research use only.
Storage Conditions
Store the TTF-1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Nuclear
Image Legend
IHC: Formalin-fixed, paraffin-embedded human lung Adenocarcinoma stained with TTF-1 antibody (NX2.1/690)
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