Fascin Antibody
Fascin/FSCN1 binds beta-catenin and colocalizes with it at the leading edges and borders of epithelial and endothelial cells. The role of FSCN1 in regulating cytoskeletal structures for the maintenance of cell adhesion, coordinating motility and invasion through interactions with signalling pathways is an active area of research especially from the cancer biology perspective. It localizes to actin-rich protrusions at the cell surface called filopodia. FSCN1 localizes to invadopodia, membrane protrusions formed at the adherent cell surface that facilitate extracellular matrix (ECM) invasion, this provide a potential molecular mechanism for how fascin increases the invasiveness of cancer cells since fascin expression is upregulated in a spectrum of cancers. [Wiki]
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT
UniProt
Q16658
Host
Mouse
Reactivity
Human, Rat
Immunogen
Full length recombinant human protein was used as the immunogen for the Fascin antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
FSCN1/418
Applications
WB, FACS, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the Fascin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Fascin antibody is available for research use only.
Storage Conditions
Store the Fascin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Location
Cytoplasmic
Image Legend
IHC: Formalin-fixed, paraffin-embedded rat brain stained with Fascin antibody (FSCN1/418)
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
Cytokeratin 8/18 (C-51), CF488A conjugate, 0.1mg/mL
CD171 / L1CAM (L1 Cell Adhesion Molecule) (UJ127), CF405S conjugate, 0.1mg/mL
TNFS15 / VEGI (Vascular Endothelial Growth Inhibitor) (rVEGI/1283), CF594 conjugate, 0.1mg/mL
Cytokeratin 16 (KRT16) (Suprabasal Keratinocyte Marker) (KRT16/2043R), CF740 conjugate, 0.1mg/mL
