Fascin Antibody
Fascin organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration. [UniProt]
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
Q16658
Host
Mouse
Reactivity
Human
Immunogen
Full length recombinant human protein was used as the immunogen for the Fascin antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
SPM133
Applications
WB, FACS, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Fascin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Fascin antibody is available for research use only.
Storage Conditions
Store the Fascin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cytoplasmic
Image Legend
IHC analysis of formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with Fascin antibody (clone SPM133) .
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